Genetic Engineering and Biotechnology Research Laboratory

Welcome To Genetic Engineering and Biotechnology Research Laboratory

Overview

Genetic Engineering and Biotechnology Research laboratory (GEBRL) was launched in Centre for Advanced Research in Sciences (CARS) in 2006. The main goal of GEBRL is advancing scientific knowledge in the field of molecular biology, genetic engineering and biotechnology especially clinical genetics through scientific research, training and practical courses for young scientific staff and postgraduate studies. This laboratory is well equipped for various molecular biology fields and all are in operation and at the "state of art condition".

Personnel:
  • Chief Scientist: Dr. Gazi Nurun Nahar Sultana (Biochemistry)
  • Senior Scientist: Dr. Rokeya Begum (Biochemistry and molecular Biology)
  • Research Fellow: Most. Umme Habiba (Biochemistry and molecular Biology)
  • Laboratory Attendant: Sharif Moshiur Rahman

Research of interest:
  • Developing Biomarker for beta thalassemia mutation detection
  • Developing molecular techniques for rapid diagnosis of beta thalassemia carrier
  • Developing biomarker for breast cancer detection in early stage
  • Detection of p53 gene mutation in gastric cancer patient
  • Population genetics to understand the genetic makeup of Bangladesh population
  • Molecular basis of type2 diabetes through responsible gene mutation
  • Molecular basis of antidiabetic drug response

Current Projects:

Title: Genetic structure of Tibeto-Burman population of Bangladesh: Estimating the gene flow along the side of Bay of Bengal
PI: Gazi Nurun Nahar Sultana (CARS, DU)
Collaborator: Professor Richard Villems (EBC)
Project Grant: Collaborative US$ 6000/year
Funding Agency: CARS, DU and Estonia Biocentre, University of Tartu
Funding year: 2014-2018

Title: Screening of β-Thalassemia HBB gene mutations for diagnosti cmarker
PI: Dr. Gazi Nurun Nahar Sultana (CARS, DU)
Co-Dr. Rokeya Begum (CARS, DU)
Grant Received: Six Lakhs Taka
Funding Agency: Ministry of Science and Technology, GOB
Funding year: 2015-2016

Title: Screening of reported OCT1 gene mutation in Bangladeshi general population
PI: Dr. Rokeya Begum, (CARS, DU)
Funding Agency: CARS, DU
Funding year: 2016-2017

Title: Molecular Mechanism of Pathogenesis linking type 2 diabetes and hypertension within Bangladeshi population
PI: Dr. Jesmin (GEB, DU)
Co-Dr. Rokeya Begum (CARS, DU)
Grant Received: Thirty Lakhs Taka
Funding Agency: Ministry of Education, GOB Funding year: 2016-2018

Success:
  • First identifying the complete spectrum of beta thalassemia mutations in Bangladeshi population
  • Developing methods for Sanger sequencing
  • Developing methods for Human DNA profiling
  • Developing DNA extraction techniques from whole blood and tissues.

Collaboration:
  • Estonia Biocentre, Estonia
  • Bangladesh Thalassemia Foundation
  • Bangladesh Institute of Research and Rehabilitation in Diabetes, Endocrine and Metabolic Disorders (BIRDEM)
  • National Institute of cancer research and hospital, Bangladesh (NICRH)

Facilities:

(i) DNA sequencing machine (ii) DNA HybiMax (iii) ProFlex PCR system (iv) PCR thermal cycler (v) PCR cabinet (vi) Refrigerated centrifuge (vii) High-speed Centrifuge(viii) Autoclave (ix) Nanodrop Spectrophotometer (x) pH meter (xi) High precision balance and Rough balance (xii) Gel Electrophoresis equipments (xiii) Gel documentation system(xiv) Incubator (xv) Dry Heat sterilizer (xvi) Water bath (xivii) Refrigerated shaker incubator (xviii)Hot plate and Magnatic Stirrer and (xix) -40°C , -20°C and normal freezers.

Services:
  • DNA Sequencing (only from purified PCR product)
  • DNA profiling
  • PCR (Instrument only)
  • DNA/RNA extraction (Space and instrument only)
  • DNA quantitation by Gel electrophoresis
  • DNA quantitation by Nanodrop spectrophotometer
  • Centrifuge

Training / Workshop:

GEBRL offers different training and workshops for scientists and students from different universities, academic institutions and companies. Such as

  • Hands on workshop on "Basics of Sanger Sequencing Method and Sequencing Data Analysis"
  • Training on "Different molecular techniques"

Recent Publications:
  • Gazi Nurun Nagar Sultana, Rokeya Begum, HosnearaAktar, Shamim Z, Rahim Ma, Chubey G. 2016.The Complete Spectrum of Beta (β) Thalassemia Mutations in Bangladeshi Population. Austin Biomarker and Diagnosis, 3:1024-1029.
  • Roy, R. R., Sultana, G. N., Begum, R., Shahinuzzaman, A. D., & Sharif, M. I. (2016). The Aspect of NK2 Transcription Factor Related Locus-5 (NKX2.5) Gene Mutations in Bangladeshi Atrial Septal Defect (ASD) patients and 2D Relationship with their Age. Mymensingh medical journa : MMJ, 25(1), 79-84.
  • Begum, R., Choudhury, S., Latif, Z. A., Sultana G. N. N. 2016. Effect of PTPRd rs17584499 C/T Polymorphism on Therapeutic Efficacy of Metformin in Bangladeshi Patients with Type 2 Diabetes. Biores Comm. 2(1), 182-188.
  • Gazi Nurun Nahar Sultana, Mohd Istiaq Sharif, Gyaneshwer Chaubey and Richard Villems. 2015. Evaluating the genetic impact of South and Southeast Asia on the peopling of Bangladesh by using 15 autosomal STRs. Legal Medicine.
  • Monika Karmin, Lauri Saag, Mario Vicente, Melissa A, Gyneshwar Chaubey, Gazi Nurun NaharSultana, Richard Villems, ToomasKivisild 2015. A recent bottleneck of Y-chromosome diversitycoincides with a global change in culture. Genome research; doi/10.1101/gr.186684.114.
  • Gazi Nurun Nahar Sultana, Rokeya Begum, M. Asaduzzaman, Tanvir Ahmed and Monira Akhtar 2015.Genetic Analysis of Y-Chromosomes 17 STR in Four Indigenous populations of Bangladesh.Bioresearch Communications; 01 (01): 32-34
  • Gazi Nurun Nahar Sultana, ADA Shahinuzzaman, Rokeya Begum, Iffat Jahan, M. Mizanur Rahman 2014. Genetic Variations of Mitochondrial Cytochrome B and Breast Cancer. AustinBiomarker and Diagnosis; 1(2): e1-e5.
  • Gazi Nurun Nahar Sultana, JannatulFerdousTuli, Rokeya Begum, and Rakesh Tamang. 2014.Mitochondrial DNA Control Region Variation from Bangladesh: Sequence Analysis for theEstablishment of a Forensic Database. Forensic Medicine and Anatomy Research (FMAR);2:95-100
  • Elias, S., Begum, R., Rahman, M. S., Ferdouse, A., Shozib, H., Ali, M. M., & Seraj, Z. (2014). Genotypic and phenotypic relatedness of a farmer-discovered variant with high-yielding rice growing in the same field. Plant Systematics and Evolution, 1-15. doi: 10.1007/s00606-014-1085-x
  • Gazi Nurun Nahar Sultana, Hasina Akhter Simol, Rokeya Begum, Zakaria Shamim, M. Anwar Hossain (2014). Status of Iron, Magnesium and Zinc in β-Thalassemia Patients of Bangladesh. Online International Interdisciplinary Research Journal, 1V (08): Page.No-76-83
  • Gazi Nurun Nahar Sultana, Atiqur Rahman M, Shahinuzzaman ADA, Abdullah ABM, Proyash Roy and Rokeya Begum (2014) Hutchinson-gilford progeria syndrome (hgps) with mitochondrial DNA (mtDNA) hv1 control region mutations. Unique journal of pharmaceutical and biological sciences. 02 (01): Page 29-32