Welcome To Genetic Engineering and Biotechnology Research Laboratory
Overview
Genetic Engineering and Biotechnology Research laboratory (GEBRL) was launched in Centre for Advanced Research in Sciences (CARS) in 2006. The main goal of GEBRL is advancing scientific knowledge in the field of molecular biology, genetic engineering and biotechnology especially clinical genetics through scientific research, training and practical courses for young scientific staff and postgraduate studies. This laboratory is well equipped for various molecular biology fields and all are in operation and at the "state of art condition".
Personnel:
- Chief Scientist: Dr. Gazi Nurun Nahar Sultana (Biochemistry)
- Senior Scientist: Dr. Rokeya Begum (Biochemistry and molecular Biology)
- Research Fellow: Most. Umme Habiba (Biochemistry and molecular Biology)
- Laboratory Attendant: Sharif Moshiur Rahman
Research of interest:
- Developing Biomarker for beta thalassemia mutation detection
- Developing molecular techniques for rapid diagnosis of beta thalassemia carrier
- Developing biomarker for breast cancer detection in early stage
- Detection of p53 gene mutation in gastric cancer patient
- Population genetics to understand the genetic makeup of Bangladesh population
- Molecular basis of type2 diabetes through responsible gene mutation
- Molecular basis of antidiabetic drug response
Current Projects:
Title: Genetic structure of Tibeto-Burman population of Bangladesh: Estimating the gene flow along the side of Bay of Bengal
PI: Gazi Nurun Nahar Sultana (CARS, DU)
Collaborator: Professor Richard Villems (EBC)
Project Grant: Collaborative US$ 6000/year
Funding Agency: CARS, DU and Estonia Biocentre, University of Tartu
Funding year: 2014-2018
Title: Screening of β-Thalassemia HBB gene mutations for diagnosti cmarker
PI: Dr. Gazi Nurun Nahar Sultana (CARS, DU)
Co-Dr. Rokeya Begum (CARS, DU)
Grant Received: Six Lakhs Taka
Funding Agency: Ministry of Science and Technology, GOB
Funding year: 2015-2016
Title: Screening of reported OCT1 gene mutation in Bangladeshi general population
PI: Dr. Rokeya Begum, (CARS, DU)
Funding Agency: CARS, DU
Funding year: 2016-2017
Title: Molecular Mechanism of Pathogenesis linking type 2 diabetes and hypertension within Bangladeshi population
PI: Dr. Jesmin (GEB, DU)
Co-Dr. Rokeya Begum (CARS, DU)
Grant Received: Thirty Lakhs Taka
Funding Agency: Ministry of Education, GOB
Funding year: 2016-2018
Success:
- First identifying the complete spectrum of beta thalassemia mutations in Bangladeshi population
- Developing methods for Sanger sequencing
- Developing methods for Human DNA profiling
- Developing DNA extraction techniques from whole blood and tissues.
Collaboration:
- Estonia Biocentre, Estonia
- Bangladesh Thalassemia Foundation
- Bangladesh Institute of Research and Rehabilitation in Diabetes, Endocrine and Metabolic Disorders (BIRDEM)
- National Institute of cancer research and hospital, Bangladesh (NICRH)
Facilities:
(i) DNA sequencing machine (ii) DNA HybiMax (iii) ProFlex PCR system (iv) PCR thermal cycler (v) PCR cabinet (vi) Refrigerated centrifuge (vii) High-speed Centrifuge(viii) Autoclave (ix) Nanodrop Spectrophotometer (x) pH meter (xi) High precision balance and Rough balance (xii) Gel Electrophoresis equipments (xiii) Gel documentation system(xiv) Incubator (xv) Dry Heat sterilizer (xvi) Water bath (xivii) Refrigerated shaker incubator (xviii)Hot plate and Magnatic Stirrer and (xix) -40°C , -20°C and normal freezers.
Services:
- DNA Sequencing (only from purified PCR product)
- DNA profiling
- PCR (Instrument only)
- DNA/RNA extraction (Space and instrument only)
- DNA quantitation by Gel electrophoresis
- DNA quantitation by Nanodrop spectrophotometer
- Centrifuge