Centre for Advanced Research in Sciences (CARS)
cars@du.ac.bd
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Analytical Services
CTCL
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CTCL Booking Application
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Name of Teacher/Person requesting for the service
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Department/Institute/Address
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Services (please select your requisition)
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Qualitative measurement of cytotoxic effect on a cancer cell line under a microscope. (the samples are needed to be sterilized)
Qualitative measurement of cytotoxic effect on a non-cancer cell line under a microscope. (sterilized samples only)
Determination of IC50 (half maximal inhibitory concentration) on a cancer cell line. (sterilized sample only)
Determination of IC50 on a non-cancer cell line. (sterilized sample only)
Determination of ED50 (Effective dose for half of maximal response), LD50 on a cell line. (sterilized sample only)
Absorbance reading @ wavelengths (200 - 999 nm.), microplates (6- to 384- well plate)
Centrifugation: (a) Swing rotor (refrigerated; max. 50ml Falcontube, 5000 rpm), (b) Fixed Rotor (max. 1.5 ml, refrigerated 50ml Falcontube,15000 rpm)
-80?C Ultralow Temperature Freezer with generator electricity backup to keep the freezing temperature always constant between -75?C and -80?C. Importantly, authority is not responsible for any kind of damage of your samples due to sudden machine failure. You have to remove the samples with short notice if servicing of the freezer is required. It will be charged every year until termination.
Other cell culture-based study
Nanopure water (Media preparation, PCR grade)
Using laboratory facilities of cell culture lab (without using consumables)
Additional services/consumables (if required)
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Sample Information
Number of Samples
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Sample ID
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Sample Type
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Sample Specification (if applicable)
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Purpose of the work
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Information Required
Which cell line you want to test
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HeLa
Vero
Sample exposure
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Add 10%
20% of sample directly
Incubation time
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24
48 h
Others (if needed)
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Category for Billing Purpose
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Category A: DU Teachers and Students doing M.S./M.Phil/PhD under D.U
Category B: Any funded project of D.U. teachers/researchers
Category C: Service to individual/consultant/institution/agency
For Contact:
Student/Person (For category A & B)
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Mobile No
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Email
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Terms and Condition
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To facilitate credit to the respective scientist, an acknowledgment/authorship must be included in a scientific article.
All the solvents and products are sterilized properly.
Appropriate control has been given. Usually, the solvent is used as a control in which the product is dissolved.
The color product is not appropriate for quantitative measurement.
Since the study is very expensive, laborious, and time-consuming, if you are not clear on this protocol, or if you have any questions/confusion, please visit the lab (CARS 605 or call 01729171710) before submitting this application. It is better if you make your study understandable to us so that we can perform it accurately in your desired way.
Please remember that after receiving your application, we will consider that you have understood our protocol and are ready to perform the study according to our protocol with the above specifications. After approval, you cannot make any further changes. Please consider it carefully.
I have read & confirm the above points.
